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Quantitative Analysis of Ingenol in Euphorbia species via Validated Isotope Dilution Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry

SYSNO ASEP	0488656
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Quantitative Analysis of Ingenol in Euphorbia species via Validated Isotope Dilution Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry
Tvůrce(i)	Béres, T. (CZ) Dragull, K. (CZ) Pospíšil, Jiří (UEB-Q)_{ORCID, RID, SAI} Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Dančák, M. (CZ) Bíba, Ondřej (UEB-Q) Tarkowski, P. (CZ) Doležal, K. (CZ) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Celkový počet autorů	9
Zdroj.dok.	Phytochemical Analysis. - : Wiley - ISSN 0958-0344 Roč. 29, č. 1 (2018), s. 23-29
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Euphorbia genus ; ingenol ; isotope-dilution method ; mass spectrometry ; ultra-high performance liquid chromatography
Vědní obor RIV	FD - Onkologie a hematologie
Obor OECD	Analytical chemistry
CEP	GA17-14007S GA ČR - Grantová agentura ČR
	LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UEB-Q - RVO:61389030
UT WOS	000416940000003
EID SCOPUS	85035794109
DOI	10.1002/pca.2711
Anotace	Introduction: Various species of the Euphorbia genus contain diterpene ingenol and ingenol mebutate (ingenol-3-angelate), a substance found in the sap of the plant Euphorbia peplus and an inducer of cell death. A gel formulation of the drug has been approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for the topical treatment of actinic keratosis. Objective: To develop a rapid and reliable method for quantification of ingenol in various plant extracts. Methodology: Methanolic extracts of 38 species of the Euphorbia genus were analysed via ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC–MS/MS) after methanolysis and solid-phase extraction (SPE) purification. The 18 O-labelled ingenol analogue was prepared and used as an internal standard for ingenol content determination and method validation. Results: The highest ingenol concentration (547 mg/kg of dry weight) was found in the lower leafless stems of E. myrsinites. The screening confirms a substantial amount of ingenol in species studied previously and furthermore, reveals some new promising candidates. Conclusion: The newly established UHPLC–MS/MS method shows to be an appropriate tool for screening of the Euphorbia genus for ingenol content and allows selection of species suitable for raw material production and/or in vitro culture initiation.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2019

Počet záznamů: 1