Počet záznamů: 1
Light influences cytokinin biosynthesis and sensing in Nostoc (cyanobacteria)
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SYSNO ASEP 0476607 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Light influences cytokinin biosynthesis and sensing in Nostoc (cyanobacteria) Tvůrce(i) Frébortová, J. (CZ)
Plíhal, O. (CZ)
Florová, P. (CZ)
Kokáš, F. (CZ)
Kubiasová, K. (CZ)
Greplová, M. (CZ)
Šimura, J. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Frébort, I. (CZ)Celkový počet autorů 9 Zdroj.dok. Journal of Phycology. - : Wiley - ISSN 0022-3646
Roč. 53, č. 3 (2017), s. 703-714Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cytokinin ; expression profiling ; miaA ; Nostoc ; RNA-seq ; sensor kinase ; tRNA IPT Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000402675300019 EID SCOPUS 85018403823 DOI 10.1111/jpy.12538 Anotace Cytokinins are an important group of plant hormones that are also found in other organisms, including cyanobacteria. While various aspects of cytokinin function and metabolism are well understood in plants, the information is limited for cyanobacteria. In this study, we first experimentally confirmed a prenylation of tRNA by recombinant isopentenyl transferase NoIPT2 from Nostoc sp. PCC 7120, whose encoding gene we previously identified in Nostoc genome along with the gene for adenylate isopentenyl transferase NoIPT1. In contrast to NoIPT2, the transcription of NoIPT1 was strongly activated during the dark period and was followed by an increase in the cytokinin content several hours later in the light period. Dominant cytokinin metabolites detected at all time points were free bases and monophosphates of isopentenyladenine and cis-zeatin, while N-glucosides were not detected at all. Whole transcriptome differential expression analysis of cultures of the above Nostoc strain treated by cytokinin compared to untreated controls indicated that cytokinin together with light trigger expression of several genes related to signal transduction, including two-component sensor histidine kinases and two-component hybrid sensors and regulators. One of the affected histidine kinases with a cyclase/histidine kinase-associated sensory extracellular domain similar to the cytokinin-binding domain in plant cytokinin receptors was able to modestly bind isopentenyladenine. The data show that the genetic disposition allows Nostoc not only to produce free cytokinins and prenylate tRNA but also modulate the cytokinin biosynthesis in response to light, triggering complex changes in sensing and regulation. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1