Počet záznamů: 1
Repetitive DNA: A Versatile Tool for Karyotyping in Festuca pratensis Huds
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SYSNO ASEP 0476564 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Repetitive DNA: A Versatile Tool for Karyotyping in Festuca pratensis Huds Tvůrce(i) Křivánková, Anna (UEB-Q)
Kopecký, David (UEB-Q) RID, ORCID
Stočes, Štěpán (UEB-Q) RID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Hřibová, Eva (UEB-Q) RID, ORCIDCelkový počet autorů 5 Zdroj.dok. Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581
Roč. 151, č. 2 (2017), s. 96-105Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Fluorescence in situ hybridization ; Karyotyping ; Meadow fescue ; Repetitive DNA ; Tandem organized repeats Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000404007600005 EID SCOPUS 85016641443 DOI 10.1159/000462915 Anotace FISH is a useful method to identify individual chromosomes in a karyotype and to discover their structural changes accompanying genome evolution and speciation. DNA probes for FISH should be chromosome specific and/or exhibit specific patterns of distribution along each chromosome. Such probes are not available in many plants including meadow fescue (Festuca pratensis Huds.), an important forage grass species. In the present study, various DNA repeats identified in Illumina shotgun sequences specific to chromosome 4F of F. pratensis were used as probes for FISH to develop the molecular karyotype of meadow fescue and to reveal a long-range molecular organization of its chromosomes. Five tandem repeats produced specific patterns on individual chromosomes. Their use in combination with probes for rRNA genes enabled the establishment of the molecular karyotype of meadow fescue. Most of the mobile genetic elements were dispersed along all the chromosomes except for the DNA transposon CACTA, which was localized preferentially to telomeric and subtelomeric regions, and a putative LTR element, which was localized to (peri)centromeric regions. Cytogenetic mapping of the 5 tandem repeats in other accessions of meadow fescue showed a highly similar distribution and confirmed the versatility and robustness of these probes. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1