Počet záznamů: 1
BODIPY-based fluorescent liposomes with sesquiterpene lactone trilobolide
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SYSNO ASEP 0476521 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název BODIPY-based fluorescent liposomes with sesquiterpene lactone trilobolide Tvůrce(i) Škorpilová, Ludmila (UEB-Q)
Rimpelová, S. (CZ)
Jurášek, M. (CZ)
Buděšínský, Miloš (UOCHB-X) RID, ORCID
Lokajová, Jana (UOCHB-X)
Effenberg, R. (CZ)
Slepička, P. (CZ)
Ruml, T. (CZ)
Kmoníčková, Eva (UEM-P) RID
Drašar, P. B. (CZ)
Wimmer, Zdeněk (UEB-Q) RID, ORCIDCelkový počet autorů 11 Zdroj.dok. Beilstein Journal of Organic Chemistry. - : Beilstein - Institut zur Foerderung der Chemischen Wissenschaften - ISSN 1860-5397
Roč. 13, JUL 4 (2017), s. 1316-1324Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova BODIPY conjugates ; Cancer targeting ; Drug delivery ; Liposomes ; Natural compounds ; Sesquiterpene lactone trilobolide Vědní obor RIV CC - Organická chemie Obor OECD Organic chemistry Vědní obor RIV – spolupráce Ústav experimentální medicíny - Farmakologie a lékárnická chemie CEP LD15012 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1304 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 ; UOCHB-X - RVO:61388963 ; UEM-P - RVO:68378041 UT WOS 000405427300001 EID SCOPUS 85023616975 DOI 10.3762/bjoc.13.128 Anotace Like thapsigargin, which is undergoing clinical trials, trilobolide is a natural product with promising anticancer and anti-inflammatory properties. Similar to thapsigargin, it has limited aqueous solubility that strongly reduces its potential medicinal applications. The targeted delivery of hydrophobic drugs can be achieved using liposome-based carriers. Therefore, we designed a traceable liposomal drug delivery system for trilobolide. The fluorescent green-emitting dye BODIPY, cholesterol and trilobolide were used to create construct 6. The liposomes were composed of dipalmitoyl-3-trimethylammoniumpropane and phosphatidylethanolamine. The whole system was characterized by atomic force microscopy, the average size of the liposomes was 150 nm in width and 30 nm in height. We evaluated the biological activity of construct 6 and its liposomal formulation, both of which showed immunomodulatory properties in primary rat macrophages. The uptake and intracellular distribution of construct 6 and its liposomal formulation was monitored by means of live-cell fluorescence microscopy in two cancer cell lines. The encapsulation of construct 6 into the liposomes improved the drug distribution in cancer cells and was followed by cell death. This new liposomal trilobolide derivative not only retains the biological properties of pure trilobolide, but also enhances the bioavailability, and thus has potential for the use in theranostic applications. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1