Počet záznamů: 1
Molecular Cytogenetic Mapping of Satellite DNA Sequences in Aegilops geniculata and Wheat
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SYSNO ASEP 0467787 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Molecular Cytogenetic Mapping of Satellite DNA Sequences in Aegilops geniculata and Wheat Tvůrce(i) Koo, D.H. (US)
Tiwari, V.K. (US)
Hřibová, Eva (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Friebe, B. (US)
Gill, B.S. (US)Celkový počet autorů 6 Zdroj.dok. Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581
Roč. 148, č. 4 (2016), s. 314-321Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova in-situ hybridization ; chromosome addition lines ; resistance genes lr57 ; repetitive dna ; triticum-ovatum ; powdery mildew ; plant genome ; bread wheat ; leaf rust ; identification ; Aegilops geniculata ; Chromosome identification ; Fluorescence in situ hybridization ; Satellite DNA ; Wheat Vědní obor RIV EB - Genetika a molekulární biologie CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000383706100010 DOI 10.1159/000447471 Anotace Fluorescence in situ hybridization (FISH) provides an efficient system for cytogenetic analysis of wild relatives of wheat for individual chromosome identification, elucidation of homoeologous relationships, and for monitoring alien gene transfers into wheat. This study is aimed at developing cytogenetic markers for chromosome identification of wheat and Aegilops geniculata (2n = 4x = 28, (UUMMg)-U-g-M-g-M-g) using satellite DNAs obtained from flow-sorted chromosome 5M(g). FISH was performed to localize the satellite DNAs on chromosomes of wheat and selected Aegilops species. The FISH signals for satellite DNAs on chromosome 5M(g) were generally associated with constitutive heterochromatin regions corresponding to C-band-positive chromatin including telomeric, pericentromeric, centromeric, and interstitial regions of all the 14 chromosome pairs of Ae. geniculata. Most satellite DNAs also generated FISH signals on wheat chromosomes and provided diagnostic chromosome arm-specific cytogenetic markers that significantly improved chromosome identification in wheat. The newly identified satellite DNA CL36 produced localized M-g genome chromosome-specific FISH signals in Ae. geniculata and in the M genome of the putative diploid donor species Ae. comosa subsp. sub-ventricosa but not in Ae. comosa subsp. comosa, suggesting that the M-g genome of Ae. geniculata was probably derived from subsp. subventricosa. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1