Počet záznamů: 1
Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus
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SYSNO ASEP 0464107 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus Tvůrce(i) Nováková, Miroslava (UEB-Q) RID
Kim, P.D. (CZ)
Šašek, Vladimír (UEB-Q) RID
Burketová, Lenka (UEB-Q) RID, ORCID
Jindřichová, Barbora (UEB-Q) RID, ORCID
Šantrůček, J. (CZ)
Valentová, O. (CZ)Zdroj.dok. Biotechnology Progress. - : Wiley - ISSN 8756-7938
Roč. 32, č. 4 (2016), s. 918-928Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova elicitor ; Brassica napus ; Leptosphaeria maculans Vědní obor RIV GF - Choroby, škůdci, plevely a ochrana rostlin CEP GA522/08/1581 GA ČR - Grantová agentura ČR QH81201 GA MZe - Ministerstvo zemědělství LD14093 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000383396800011 DOI 10.1002/btpr.2266 Anotace The Dothideomycete Leptosphaeria maculans, a worldwide fungal pathogen of oilseed rape (Brassica napus), secretes a broad spectrum of molecules into the cultivation medium during growth in vitro. Here, candidate elicitor molecules, which induce resistance in B. napus to L. maculans, were identified in the cultivation medium. The elicitation activity was indicated by increased transcription of pathogenesis-related gene 1 (PR1) and enhanced resistance of B. napus plants to the invasion of L. maculans. The elicitation activity was significantly lowered when the cultivation medium was heated to 80 degrees C. Active components were further characterized by specific cleavage with the proteolytic enzymes trypsin and proteinase K and with glycosidases -amylase and -glucanase. The elicitor activity was eliminated by proteolytic digestion while glycosidases had no effect. The filtered medium was fractionated by either ion-exchange chromatography or isoelectric focusing. Mass spectrometry analysis of the most active fractions obtained by both separation procedures revealed predominantly enzymes that can be involved in the degradation of plant cell wall polysaccharides. This is the first study searching for L. maculans-specific secreted elicitors with a potential to be used as defense-activating agents in the protection of B. napus against L. maculans in agriculture. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1