Počet záznamů: 1
PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis
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SYSNO ASEP 0462665 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis Tvůrce(i) Simon, S. (DE)
Skůpa, Petr (UEB-Q) RID
Viaene, T. (BE)
Zwiewka, M. (CZ)
Tejos, R. (BE)
Klíma, Petr (UEB-Q) RID, ORCID
Čarná, Mária (UEB-Q)
Rolčík, J. (CZ)
De Rycke, R. (BE)
Moreno, I. (ES)
Dobrev, Petre (UEB-Q) RID, ORCID
Orellana, A. (CL)
Zažímalová, Eva (UEB-Q) RID, ORCID
Friml, J. (AT)Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 211, č. 1 (2016), s. 65-74Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova auxin ; endoplasmic reticulum (ER) ; lateral root Vědní obor RIV ED - Fyziologie CEP ED1.1.00/02.0068 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA16-10948S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000379212800008 DOI 10.1111/nph.14019 Anotace Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate.
In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles.
The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes.
These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1