Počet záznamů: 1
Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy
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SYSNO ASEP 0461678 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy Tvůrce(i) Laňková, Martina (UEB-Q) RID, ORCID
Humpolíčková, Jana (UFCH-W) RID
Vosolsobě, S. (CZ)
Cit, Zdeněk (UEB-Q)
Lacek, Jozef (UEB-Q) ORCID
Čovan, Martin (UEB-Q)
Čovanová, Milada (UEB-Q) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCIDZdroj.dok. Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 22, č. 2 (2016), s. 290-299Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova raster image correlation spectroscopy ; fluorescence recovery after photobleaching ; auxin influx Vědní obor RIV EB - Genetika a molekulární biologie Vědní obor RIV – spolupráce Ústav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie CEP GAP305/11/2476 GA ČR - Grantová agentura ČR GPP501/12/P951 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 ; UFCH-W - RVO:61388955 UT WOS 000378274600005 DOI 10.1017/S1431927616000568 Anotace A number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more widely used fluorescence recovery after photobleaching method. For RICS, a set of microscopy images is obtained by single-photon confocal laser scanning microscopy (CLSM). Fluorescence fluctuations are subsequently correlated between individual pixels and the information on protein mobility are extracted using a model that considers processes generating the fluctuations such as diffusion and chemical binding reactions. As we show here using an example of two integral PM transporters of the plant hormone auxin, RICS uncovered their distinct short-distance lateral mobility within the PM that is dependent on cytoskeleton and sterol composition of the PM. RICS, which is routinely accessible on modern CLSM instruments, thus represents a valuable approach for studies of dynamics of PM proteins in plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1