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Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress
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SYSNO ASEP 0461675 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress Tvůrce(i) Pospíšilová, H. (CZ)
Jiskrová, E. (CZ)
Vojta, P. (CZ)
Mrízová, K. (CZ)
Kokáš, F. (CZ)
Majeská Čudějková, M. (CZ)
Bergougnoux, V. (CZ)
Plíhal, O. (CZ)
Klimešová, J. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Dzurová, L. (CZ)
Frébort, I. (CZ)
Galuszka, P. (CZ)Zdroj.dok. New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 33, č. 5 (2016), s. 692-705Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova ROOT-GROWTH ; OXIDASE/DEHYDROGENASE GENES ; BETA-GLUCOSIDASE Vědní obor RIV EB - Genetika a molekulární biologie CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000378026400011 DOI 10.1016/j.nbt.2015.12.005 Anotace Together with auxins, cytokinins are the main plant hormones involved in many different physiological processes. Given this knowledge, cytokinin levels can be manipulated by genetic modification in order to improve agronomic parameters of cereals in relation to, for example, morphology, yield, and tolerance to various stresses. The barley (Hordeum vulgare) cultivar Golden Promise was transformed using the cytokinin dehydrogenase 1 gene from Arabidopsis thaliana (AtCKX1) under the control of mild root-specific beta-glucosidase promoter from maize. Increased cytokinin degradation activity was observed positively to affect the number and length of lateral roots. The impact on morphology depended upon the recombinant protein's subcellular compartmentation. While assumed cytosolic and vacuolar targeting of AtCKX1 had negligible effect on shoot growth, secretion of AtCKX1 protein to the apoplast had a negative effect on development of the aerial part and yield. Upon the application of severe drought stress, all transgenic genotypes maintained higher water content and showed better growth and yield parameters during revitalization. Higher tolerance to drought stress was most caused by altered root morphology resulting in better dehydration avoidance. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
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