Počet záznamů: 1
Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1
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SYSNO ASEP 0447821 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1 Tvůrce(i) Bouguyon, E. (FR)
Brun, F. (FR)
Meynard, D. (FR)
Kubeš, Martin (UEB-Q) RID, ORCID
Pervent, M. (FR)
Leran, S. (FR)
Lacombe, B. (FR)
Krouk, G. (FR)
Guiderdoni, E. (FR)
Zažímalová, Eva (UEB-Q) RID, ORCID
Hoyerová, Klára (UEB-Q) RID, ORCID
Nacry, P. (FR)
Gojon, A. (FR)Zdroj.dok. Nature Plants - ISSN 2055-026X
Roč. 1, March (2015), s. 15015Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova nitrate transceptor ; Arabidopsis ; lateral root development Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/11/0797 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000364391800002 DOI 10.1038/NPLANTS.2015.15 Anotace In Arabidopsis the plasma membrane nitrate transceptor (transporter/receptor) NRT1.1 governs many physiological and developmental responses to nitrate. Alongside facilitating nitrate uptake, NRT1.1 regulates the expression levels of many nitrate assimilation pathway genes, modulates root system architecture, relieves seed dormancy and protects plants from ammonium toxicity. Here, we assess the functional and phenotypic consequences of point mutations in two key residues of NRT1.1 (P492 and T101). We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, and its longer-term downregulation, at high nitrate concentrations. We also show that these mutations have differential effects on genome-wide gene expression. Our findings indicate that NRT1.1 activates four separate signalling mechanisms, which have independent structural bases in the protein. In particular, we present evidence to suggest that the phosphorylated and non-phosphorylated forms of NRT1.1 at T101 have distinct signalling functions, and that the nitrate-dependent regulation of root development depends on the phosphorylated form. Our findings add to the evidence that NRT1.1 is able to trigger independent signalling pathways in Arabidopsis in response to different environmental conditions. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2016
Počet záznamů: 1