Počet záznamů: 1

Involvement of the Iron Regulatory Protein from Eisenia andrei Earthworms in the Regulation of Cellular Iron Homeostasis

SYSNO ASEP	0435252
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Involvement of the Iron Regulatory Protein from Eisenia andrei Earthworms in the Regulation of Cellular Iron Homeostasis
Tvůrce(i)	Procházková, Petra (MBU-M)_{RID, ORCID} Škanta, František (MBU-M)_RID Roubalová, Radka (MBU-M)_{RID, ORCID} Šilerová, Marcela (MBU-M) Dvořák, Jiří (MBU-M)_{RID, ORCID} Bilej, Martin (MBU-M)_{RID, ORCID}
Zdroj.dok.	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 9, č. 10 (2014)
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	MULTIPLE SEQUENCE ALIGNMENT ; ELEMENT-BINDING PROTEIN ; FERRITIN MESSENGER-RNA
Vědní obor RIV	EE - Mikrobiologie, virologie
CEP	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EE2.3.20.0055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000342670800103
DOI	10.1371/journal.pone.0109900
Anotace	Iron homeostasis in cells is regulated by iron regulatory proteins (IRPs) that exist in different organisms. IRPs are cytosolic proteins that bind to iron-responsive elements (IREs) of the 5'- or 3'-untranslated regions (UTR) of mRNAs that encode many proteins involved in iron metabolism. In this study, we have cloned and described a new regulatory protein belonging to the family of IRPs from the earthworm Eisenia andrei (EaIRP). The earthworm IRE site in 5'-UTR of ferritin mRNA most likely folds into a secondary structure that differs from the conventional IRE structures of ferritin due to the absence of a typically unpaired cytosine that participates in protein binding. Prepared recombinant EaIRP and proteins from mammalian liver extracts are able to bind both mammalian and Eisenia IRE structures of ferritin mRNA, although the affinity of the rEaIRP/Eisenia IRE structure is rather low. This result suggests the possible contribution of a conventional IRE structure. When IRP is supplemented with a Fe-S cluster, it can function as a cytosolic aconitase. Cellular cytosolic and mitochondrial fractions, as well as recombinant EaIRP, exhibit aconitase activity that can be abolished by the action of oxygen radicals. The highest expression of EaIRP was detected in parts of the digestive tract. We can assume that earthworms may possess an IRE/IRP regulatory network as a potential mechanism for maintaining cellular iron homeostasis, although the aconitase function of EaIRP is most likely more relevant.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2015

Počet záznamů: 1