Počet záznamů: 1

UHPLC-MS/MS based target profiling of stress-induced phytohormones

SYSNO ASEP	0433242
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	UHPLC-MS/MS based target profiling of stress-induced phytohormones
Tvůrce(i)	Floková, Kristýna (UEB-Q)_{ORCID, RID} Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Miersch, Otto (UEB-Q)_{RID, SAI} Strnad, Miroslav (UEB-Q)_{RID, ORCID} Wasternack, Claus (UEB-Q)_ORCID Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI}
Zdroj.dok.	Phytochemistry. - : Elsevier - ISSN 0031-9422 Roč. 105, SEP 2014 (2014), s. 147-157
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Stress-induced phytohormones ; Jasmonates ; Abscisic acid
Vědní obor RIV	CB - Analytická chemie, separace
CEP	GA14-34792S GA ČR - Grantová agentura ČR
	LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LK21306 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UEB-Q - RVO:61389030
UT WOS	000340986900019
DOI	10.1016/j.phytochem.2014.05.015
Anotace	Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight. FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2015

Počet záznamů: 1