Počet záznamů: 1
N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant
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SYSNO ASEP 0431025 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant Tvůrce(i) Sieper, T. (DE)
Forczek, Sándor (UEB-Q) RID, ORCID
Matucha, Miroslav (UEB-Q)
Kraemer, P. (DE)
Hartmann, A. (DE)
Schroeder, P. (DE)Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 201, č. 2 (2014), s. 545-555Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova barley (Hordeum vulgare) ; monoclonal antibodies ; N-acyl-homoserine lactones (HSLs) Vědní obor RIV EF - Botanika Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000328511700019 DOI 10.1111/nph.12519 Anotace Bacteria communicate with each other in a population density-dependent process known as quorum sensing. N-acyl-homoserine lactones (HSLs) are the autoinducers of Gram-negative bacteria and the best-studied quorum sensing signals so far. HSLs induce various responses in plants, including systemic resistance and root development. Here, we used different methods, including tritium labelling, sensor strain assays and monoclonal antibodies (mAbs), to analyse the uptake and translocation of C8- and C10- homoserine lactones into barley (Hordeum vulgare cv Barke). Both HSLs were already systemically transported into the shoot at 2h after application. HSL uptake could be inhibited by orthovanadate, demonstrating that ABC transporters are involved in the uptake. Root transport occurs predominantly via the central cylinder, which was shown by transport inhibition via KCl application and autoradiography of root cross-sections. Furthermore, a newly established detection method with mAbs allowed the first detection of a systemic transport of long-chain HSLs in plants. The coupled use of different HSL detection methods demonstrated that the uptake and transport of HSLs into barley does not occur passively, but relies, at least partially, on active processes in the plant. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015
Počet záznamů: 1