Počet záznamů: 1
Translation Initiation Factors eIF3 and HCR1 Control Translation Termination and Stop Codon Read-Through in Yeast Cells
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SYSNO ASEP 0425640 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Translation Initiation Factors eIF3 and HCR1 Control Translation Termination and Stop Codon Read-Through in Yeast Cells Tvůrce(i) Beznosková, Petra (MBU-M) RID
Cuchalová, Lucie (MBU-M)
Wagner, Susan (MBU-M) RID, ORCID
Shoemaker, Ch. J. (US)
Gunišová, Stanislava (MBU-M) RID
von der Haar, T. (GB)
Valášek, Leoš Shivaya (MBU-M) RID, ORCIDZdroj.dok. PLoS Genetics. - : Public Library of Science - ISSN 1553-7404
Roč. 9, č. 11 (2013)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova RNA RECOGNITION MOTIF ; MESSENGER-RNA ; IN-VIVO Vědní obor RIV EB - Genetika a molekulární biologie CEP GBP305/12/G034 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000330369000042 DOI 10.1371/journal.pgen.1003962 Anotace Translation is divided into initiation, elongation, termination and ribosome recycling. Earlier work implicated several eukaryotic initiation factors (eIFs) in ribosomal recycling in vitro. Here, we uncover roles for HCR1 and eIF3 in translation termination in vivo. A substantial proportion of eIF3, HCR1 and eukaryotic release factor 3 (eRF3) but not eIF5 (a well-defined "initiation-specific" binding partner of eIF3) specifically co-sediments with 80S couples isolated from RNase-treated heavy polysomes in an eRF1-dependent manner, indicating the presence of eIF3 and HCR1 on terminating ribosomes. eIF3 and HCR1 also occur in ribosome-and RNA-free complexes with both eRFs and the recycling factor ABCE1/RLI1. Several eIF3 mutations reduce rates of stop codon read-through and genetically interact with mutant eRFs. In contrast, a slow growing deletion of hcr1 increases read-through and accumulates eRF3 in heavy polysomes in a manner suppressible by overexpressed ABCE1/RLI1. Based on these and other findings we propose that upon stop codon recognition, HCR1 promotes eRF3.GDP ejection from the post-termination complexes to allow binding of its interacting partner ABCE1/RLI1 Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
Počet záznamů: 1