Počet záznamů: 1
Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus
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SYSNO ASEP 0397255 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus Tvůrce(i) Kim, P.D. (CZ)
Šašek, Vladimír (UEB-Q) RID
Burketová, Lenka (UEB-Q) RID, ORCID
Čopíková, J. (CZ)
Synytsya, A. (CZ)
Jindřichová, Barbora (UEB-Q) RID, ORCID
Valentová, O. (CZ)Zdroj.dok. Journal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561
Roč. 61, č. 22 (2013), s. 5207-5214Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova elicitor ; oligosaccharide ; Leptosphaeria maculans Vědní obor RIV GF - Choroby, škůdci, plevely a ochrana rostlin CEP GA522/08/1581 GA ČR - Grantová agentura ČR QH81201 GA MZe - Ministerstvo zemědělství CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000320153500003 DOI 10.1021/jf401221v Anotace Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with alpha- or beta-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (similar to 58 mol %), mannose (similar to 22 mol %), and galactose (similar to 18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue alpha-D-Glcp-(1 -> 2)-beta-D-Galf-(1 -> 6)-alpha-D-Manp-(1 -> 3)-L-Ser. Terminal alpha-D-Glcp and (1 -> 6)-beta-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrated induce defence response in B. napus plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2014
Počet záznamů: 1