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Corynebacterium glutamicum promoters: a practical approach
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SYSNO ASEP 0396036 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Corynebacterium glutamicum promoters: a practical approach Tvůrce(i) Pátek, Miroslav (MBU-M) RID, ORCID
Holátko, Jiří (MBU-M)
Busche, T. (DE)
Kalinowski, J. (DE)
Nešvera, Jan (MBU-M) RIDZdroj.dok. Microbial Biotechnology - ISSN 1751-7907
Roč. 6, č. 2 (2013), s. 103-117Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova VITRO TRANSCRIPTION SYSTEM ; L-LYSINE PRODUCTION ; SIGMA-FACTOR SIGB Vědní obor RIV EE - Mikrobiologie, virologie CEP GPP302/12/P633 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000315124400002 DOI 10.1111/1751-7915.12019 Anotace Transcription initiation is the key step in gene expression in bacteria, and it is therefore studied for both theoretical and practical reasons. Promoters, the traffic lights of transcription initiation, are used as construction elements in biotechnological efforts to coordinate green waves' in the metabolic pathways leading to the desired metabolites. Detailed analyses of Corynebacterium glutamicum promoters have already provided large amounts of data on their structures, regulatory mechanisms and practical capabilities in metabolic engineering. In this minireview the main aspects of promoter studies, the methods developed for their analysis and their practical use in C.glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C.glutamicum promoter studies are outlined Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
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