Počet záznamů: 1
Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed
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SYSNO ASEP 0395044 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed Tvůrce(i) Guzy-Wrobelska, J. (PL)
Filek, M. (PL)
Kaliciak, A. (PL)
Szarejko, I. (PL)
Macháčková, Ivana (UEB-Q)
Krekule, Jan (UEB-Q)
Barciszewska, M. (PL)Zdroj.dok. Acta Physiologiae Plantarum. - : Springer - ISSN 0137-5881
Roč. 35, č. 3 (2013), s. 817-827Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova Brassica napus ; DNA methylation ; MSAP Vědní obor RIV EF - Botanika CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000315035000019 DOI 10.1007/s11738-012-1126-4 Anotace Vernalization-induced flowering is an effect of the epigenetic regulation of gene expression through DNA methylation and histone modifications. Vernalization-mediated silencing of a floral repressor through histone modifications was shown in Arabidopsis thaliana. However, for Brassica napus L., the mechanism underlying vernalization is unclear, and the roles of DNA methylation and histone modifications have not been established. This study revealed the profiles of changes in the DNA methylation state during vernalization (after 14, 35, 56 days) and the subsequent growth in long- or short-day photoperiods (after 2, 7, 14 days) in the winter and spring rapeseed using TLC and MSAP techniques. TLC analysis showed a significant decrease in the amount of 5-methylcytosine (m(5)C) in genomic DNA in both cultivars at the beginning of vernalization, but upon its termination, the winter rape showed a reduced level of m(5)C contrary to a significantly increased level in the spring rape. MSAP analysis revealed that winter and spring rapeseed differed in the MSAP loci which were demethylated/methylated in the course of the experiment and presented diverse profiles of changes in the methylation state. The winter rape showed permanent demethylations at 69.2 % of MSAP loci in the course of vernalization that were mostly preserved upon its termination. The spring rape showed similar numbers of demethylations and methylations that were mainly transient. The study provides evidence of the role of DNA methylation in vernalization for rapeseed and for the significant prevalence of demethylations at the beginning of vernalization, which is necessary for the transition to reproductive growth. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2014
Počet záznamů: 1