Počet záznamů: 1
Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots
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SYSNO ASEP 0382535 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots Tvůrce(i) Langhansová, Lenka (UEB-Q) RID, ORCID
Maršík, Petr (UEB-Q) RID, ORCID
Vaněk, Tomáš (UEB-Q) RID, ORCIDZdroj.dok. Industrial Crops and Products. - : Elsevier - ISSN 0926-6690
Roč. 35, č. 1 (2012), s. 154-159Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Adventitious roots ; Panax ginseng ; Ginsenosides Vědní obor RIV CE - Biochemie CEP KJB400550705 GA AV ČR - Akademie věd ME08070 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000296110700021 DOI 10.1016/j.indcrop.2011.06.028 Anotace Differentiated tissue in Panax ginseng cultures was found to be very efficacious for saponin production. In order to increase the yield of saponins and preserve culture stability we were testing different plant growth regulators (PGR) and auxin/cytokinin combinations to regulate a level of tissue differentiation. For this purpose we used transverse thin cell layers (tTCLs) of adventitious roots of Panax ginseng. Adventitious roots were cultivated in Shenk and Hildebrand (SH) liquid medium supplemented with IBA (24.6 mu M). Callus formation and root multiplication of adventitious root tTCLs was evaluated after 4 and following 12 weeks of cultivation, respectively, on SH basal medium containing various auxins (3 mg l(-1)) or cytokinins (0.2 or 0.02 mg l(-1)) or their combinations. We found that kinetin (Kin) in combination with auxin benzo[b]selenienyl acetic acid (BSAA), naphthalene acetic acid or indole-3-butric acidis the best for biomass production and following root multiplication. These combinations were tested in previously selected most suitable large-scale system a temporary immersion system RITA. The best saponin production (15.94 +/- 1.89 mg g(-1) dry weight) and growth value (5.62 +/- 0.34) was reached on medium containing BSAA and Kin combination. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1