Počet záznamů: 1
The Physiology and Proteomics of Drought Tolerance in Maize: Early Stomatal Closure as a Cause of Lower Tolerance to Short-Term Dehydration?
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SYSNO ASEP 0382487 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Physiology and Proteomics of Drought Tolerance in Maize: Early Stomatal Closure as a Cause of Lower Tolerance to Short-Term Dehydration? Tvůrce(i) Benešová, M. (CZ)
Holá, D. (CZ)
Fischer, L. (CZ)
Jedelsky, P. L. (CZ)
Hnilička, F. (CZ)
Wilhelmová, Naděžda (UEB-Q) RID, ORCID
Rothová, O. (CZ)
Kočová, M. (CZ)
Procházková, Dagmar (UEB-Q) RID
Honnerová, J. (CZ)
Fridrichová, L. (CZ)
Hniličková, H. (CZ)Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 7, č. 6 (2012), "e38017"Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova ZEA-MAYS L. ; WATER-STRESS ; GLUTATHIONE-REDUCTASE Vědní obor RIV ED - Fyziologie CEP GA521/07/0470 GA ČR - Grantová agentura ČR LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000305341900017 DOI 10.1371/journal.pone.0038017 Anotace Understanding the response of a crop to drought is the first step in the breeding of tolerant genotypes. In our study, two maize (Zea mays L.) genotypes with contrasting sensitivity to dehydration were subjected to moderate drought conditions. The subsequent analysis of their physiological parameters revealed a decreased stomatal conductance accompanied by a slighter decrease in the relative water content in the sensitive genotype. In contrast, the tolerant genotype maintained open stomata and active photosynthesis, even under dehydration conditions. Drought-induced changes in the leaf proteome were analyzed by two independent approaches, 2D gel electrophoresis and iTRAQ analysis, which provided compatible but only partially overlapping results. Drought caused the up-regulation of protective and stress-related proteins (mainly chaperones and dehydrins) in both genotypes. The differences in the levels of various detoxification proteins corresponded well with the observed changes in the activities of antioxidant enzymes. The number and levels of up-regulated protective proteins were generally lower in the sensitive genotype, implying a reduced level of proteosynthesis, which was also indicated by specific changes in the components of the translation machinery. Based on these results, we propose that the hypersensitive early stomatal closure in the sensitive genotype leads to the inhibition of photosynthesis and, subsequently, to a less efficient synthesis of the protective/detoxification proteins that are associated with drought tolerance. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1