Počet záznamů: 1
Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves
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SYSNO ASEP 0381049 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves Tvůrce(i) Behr, M. (DE)
Motyka, Václav (UEB-Q) RID, ORCID
Weihmann, F. (DE)
Malbeck, Jiří (UEB-Q) RID, ORCID
Deising, H. B. (DE)
Wirsel, S. G. R. (DE)Zdroj.dok. Molecular Plant-Microbe Interactions. - : American Phytopathological Society - ISSN 0894-0282
Roč. 25, č. 8 (2012), s. 1073-1082Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Colletotrichum graminicola ; maize ; cytokinin Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP506/11/0774 GA ČR - Grantová agentura ČR LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000306116300006 DOI 10.1094/MPMI-01-12-0012-R Anotace When inoculated onto maize leaves at the onset of senescence, the hemibiotroph Colletotrichum graminicola causes green islands that are surrounded by senescing tissue. Taking advantage of green islands as indicators of sites of the establishment of successful infection and of advanced high-performance liquid chromatography tandem mass spectrometry methodology, we analyzed changes in the patterns and levels of cytokinins (CK) at high spatial and analytical resolution. Twenty individual CK were detected in green islands. Levels of cis-zeatin-9-riboside and cis-zeatin-9-riboside-5'-monophosphate increased drastically, whereas that of the most prominent CK, cis-zeatin-O-glucoside, decreased. The fungus likely performed these conversions because corresponding activities were also detected in in vitro cultures amended with CK. We found no evidence that C. graminicola is able to synthesize CK entirely de novo in minimal medium but, after adding dimethylallyl diphosphate, a precursor of CK biosynthesis occurring in plants, a series of trans-zeatin isoforms (i.e., trans-zeatin-9-riboside-5'-monophosphate, trans-zeatin-9-riboside, and trans-zeatin) was formed. After applying CK onto uninfected leaves, transcripts of marker genes for senescence, photosynthesis, and assimilate distribution were measured by quantitative reverse-transcribed polymerase chain reaction; furthermore, pulse-amplitude modulation chlorophyll fluorometry and single-photon avalanche diode analyses were conducted. These experiments suggested that modulation of CK metabolism at the infection site affects host physiology. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1