Počet záznamů: 1

Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants

SYSNO ASEP	0380685
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants
Tvůrce(i)	Čeřovská, Noemi (UEB-Q)_{RID, ORCID} Hoffmeisterová, Hana (UEB-Q)_RID Moravec, Tomáš (UEB-Q)_{RID, ORCID} Plchová, Helena (UEB-Q)_{RID, ORCID} Folwarczna, Jitka (UEB-Q) Synková, Helena (UEB-Q)_{RID, ORCID} Ryšlavá, H. (CZ) Ludvíková, V. (CZ) Šmahel, M. (CZ)
Zdroj.dok.	Journal of Biosciences. - : Indian Academy of Sciences - ISSN 0250-5991 Roč. 37, č. 1 (2012), s. 125-133
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	IN - Indie
Klíč. slova	Human papillomavirus (HPV-16) ; L2-and E7-derived epitopes ; transient expression
Vědní obor RIV	FD - Onkologie a hematologie
CEP	GA521/06/0973 GA ČR - Grantová agentura ČR
	GA521/09/1525 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000301163400015
DOI	10.1007/s12038-011-9177-z
Anotace	Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of immunogens that can be used for development of a new generation of vaccines against a variety of infectious diseases. In this study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The protein L2108-120-PVX CP was expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The protein PVX CP-L2108-120 was expressed using mutated vector to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2013

Počet záznamů: 1