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Identification of Staphylococcus spp. isolated from ixodid ticks
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SYSNO ASEP 0315074 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Identification of Staphylococcus spp. isolated from ixodid ticks Překlad názvu Identifikace stafylokoků izolovaných z klíšťat Tvůrce(i) Švec, P. (CZ)
Nováková, D. (CZ)
Rudolf, Ivo (UBO-W) RID, ORCID, SAI
Šikutová, Silvie (UBO-W) RID, SAI, SAI, ORCID
Masaříková, J. (CZ)
Hubálek, Zdeněk (UBO-W) RID, SAI, ORCID
Sedláček, I. (CZ)Zdroj.dok. XII. International Congress of Bacteriology and Applied Microbiology, 5-9 August 2008, Istanbul: Abstract Book. - Istanbul : International Union of Microbiological Societies, 2008
S. 268Poč.str. 1 s. Akce International Congress of Bacteriology and Applied Microbiology /12./ Datum konání 05.08.2008-09.08.2008 Místo konání Istanbul Země TR - Turecko Typ akce WRD Jazyk dok. eng - angličtina Země vyd. TR - Turecko Klíč. slova ticks ; diversity ; microorganisms ; rep-PCR ; staphylococci Vědní obor RIV EE - Mikrobiologie, virologie CEP KJB600930613 GA AV ČR - Akademie věd CEZ AV0Z60930519 - UBO-W (2005-2011) Anotace A group of eight Gram-positive, catalase positive cocci was isolated during analysis of cultivable microflora of ixodid ticks, namely Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna, vectors of many vertebrate pathogens. Analysed strains were isolated from whole-body homogenized surface-sterilized ticks inoculated on different kinds of media: Tryptone Soya Agar, Brain-Heart Infusion Agar, Columbia Blood Agar and Mac Conkey Agar. Individual colonies were picked up, purified and preliminary identified by using rep-PCR fingerprinting with the primer (GTG)5. This method assigned three strains as Staphylococcus aureus subsp. aureus (DR11, HC37, HC48), two strains as Staphylococcus warneri (HC13, HC36) and the remaining strains represented Staphylococcus cohnii subsp. cohnii (DR8), Staphylococcus epidermidis (HC34) and Staphylococcus capitis subsp. capitis (HC31). These results were verified by automated ribotyping with EcoRI (RiboPrinter® microbial characterization system) as well as whole-cell protein fingerprinting and confirmed the (GTG)5-PCR fingerprinting as a fast and reliable tool for identification of staphylococci. Biotyping using API ID 32 Staph kit misidentified S. cohnii subsp. cohnii (DR8) as Staphylococcus xylosus and S. warneri (HC13) as Staphylococcus haemolyticus. Two strains (DR11 and DR8) originated from Dermacentor reticulatus and the remaining strains were isolated from Haemaphysalis concinna. Interestingly, no staphylococci were isolated from Ixodes ricinus in our study. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2009
Počet záznamů: 1