Počet záznamů: 1  

The Effect of Electric Field on Callus Induction with Rape Hypocotyls

    1.
    SYSNO ASEP0030609
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JOstatní články
    NázevThe Effect of Electric Field on Callus Induction with Rape Hypocotyls
    Překlad názvuVliv elektrického pole na indukci kalusu u hypokotylů řepky
    Tvůrce(i) Filek, M. (PL)
    Holda, M. (PL)
    Macháčková, Ivana (UEB-Q)
    Krekule, Jan (UEB-Q)
    Zdroj.dok.Zeitschrift fuer Naturforschung. Section C: a Journal of Biosciences. - : Walter de Gruyter - ISSN 0939-5075
    60c, 11/12 (2005), s. 876-882
    Poč.str.7 s.
    Jazyk dok.eng - angličtina
    Země vyd.DE - Německo
    Klíč. slovaElectric Field ; Rape ; Growth Regulators
    Vědní obor RIVEF - Botanika
    CEZAV0Z50380511 - UEB-Q (2005-2011)
    AnotaceThe influence of electric field treatment on dedifferentiation and calli formation on rape hypocotyls was investigated. Segments, 10 mm long, of the upper part of rape (Brassica napus L., cv. Go´ rcza∆ski) hypocotyls were stimulated by different combinations of voltage/time (1.5 V/120 h, 3 V/3 h, 10 V/15 min and 30 V/30 s) under in vitro conditions. With all electric field treatments, segments oriented with their apical part towards the cathode produced more calli as compared to control (non-treated with electric field). Under opposite orientation slight inhibition of callus growth was observed. As the strongest effect on callus growth was observed after treatment with 30 V/30 s, this electric field treatment was selected for following analyses: the incorporation of [14C]-2,4-D (2,4-dichlorophenoxyacetic acid) and [14C]- BAP (benzylaminopurine) from the culture medium, changes in ACC (1-aminocyclopropane- 1-carboxylic acid) level and the redox activity in apical and bottom parts of hypocotyls during 18 d of culture. Electric field treatment (30 V/30 s) stimulated a higher accumulation of 2,4-D and BAP in basal parts of hypocotyls. Moreover, orienting the apical part towards the cathode resulted in lower uptake of hormones as compared with the opposite orientation. The ACC concentration increased, especially in the basal parts of hypocotyls, independently on electric field application. The distribution of oxidative substances was not changed when the apical parts were oriented towards the cathode. Opposite orientation influenced the redistribution of oxidative substances from the first day of electric field treatment. Based on these results we suggest that electric field action can be connected with its influence on specific concentration of oxidative substances and hormone distribution in cells.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2006
Počet záznamů: 1  

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