Počet záznamů: 1
Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro
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SYSNO ASEP 0585295 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro Tvůrce(i) Rosenbaum Bartková, Alexandra (UZFG-Y) ORCID
Němcová, Lucie (UZFG-Y) RID
Strejček, F. (SK)
Gad, A. (US)
Kinterová, Veronika (UZFG-Y) ORCID
Morovič, M. (SK)
Benc, M. (SK)
Procházka, Radek (UZFG-Y) RID, ORCID
Laurinčik, J. (SK)Celkový počet autorů 9 Číslo článku 7081 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 14, č. 1 (2024)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova gene-expression ; growth-factor ; oocytes ; maturation ; fertilization Obor OECD Developmental biology CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA23-05108S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001191196900011 EID SCOPUS 85188512088 DOI 10.1038/s41598-024-57865-7 Anotace In this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium. In addition, immunocytochemical labelling confirmed the detection of UBF in 4-cell FLI parthenogenic embryos, suggesting similarities with natural embryo development. Through RNAseq analysis, upregulated genes present in 4-cell FLI embryos were found to play key roles in important biological processes such as cell proliferation, cell differentiation, and transcriptional regulation. Based on our findings, we demonstrated the positive influence of FLI medium in the evaluation of in vitro embryo production, EGA detection, transcriptomic and proteomic profile, which was confirmed by the positive activation of the embryonal genome in the 4-cell stage of parthenogenetically activated embryos. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2025 Elektronická adresa https://www.nature.com/articles/s41598-024-57865-7
Počet záznamů: 1