Počet záznamů: 1
Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells
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SYSNO ASEP 0584751 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells Tvůrce(i) Pourali, Parastoo (MBU-M)
Svoboda, Milan (UIACH-O) RID, ORCID
Neuhoferová, Eva (MBU-M) ORCID
Dzmitruk, Volha (BTO-N)
Benson, Veronika (MBU-M) RID, ORCIDZdroj.dok. Biotechnology and Applied Biochemistry. - : Wiley - ISSN 0885-4513
(2024)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cellular uptake ; size ; lipopolysaccharide ; oxide ; actin reorganization ; biologically produced gold nanoparticles ; nanoparticle uptake ; RAW264.7 cells ; stress response Obor OECD Microbiology Vědní obor RIV – spolupráce Ústav analytické chemie - Analytická chemie, separace CEP EF18_046/0015974 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023042 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EH22_010/0002357 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CIISB III - 90242 - Masarykova univerzita / Středoevropský technologický institut
Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i.Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; UIACH-O - RVO:68081715 ; BTO-N - RVO:86652036 UT WOS 001184114000001 EID SCOPUS 85188097182 DOI 10.1002/bab.2575 Anotace The biologically produced gold nanoparticles (AuNPs) are novel carriers with promising use in targeted tumor therapy. Still, there are no studies regarding the efficacy of nanoparticle internalization by cancer and noncancer cells. In this study, AuNPs were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDS), and Zetasizer. Obtained AuNPs were about 15 nm in size with a zeta potential of35.8 mV. The AuNPs were added to cancer cells (4T1), noncancer cells (NIH/3T3), and macrophages (RAW264.7). The viability decreased in 4T1 (77 +/- 3.74%) in contrast to NIH/3T3 and RAW264.7 cells (89 +/- 4.9% and 90 +/- 3.5%, respectively). The 4T1 cancer cells also showed the highest uptake and accumulation of Au (similar to 80% of AuNPs was internalized) as determined by graphite furnace atomic absorption spectroscopy. The lowest amount of AuNPs was internalized by the NIH/3T3 cells (similar to 30%). The NIH/3T3 cells exhibited prominent reorganization of F-actin filaments as examined by confocal microscopy. In RAW264.7, we analyzed the release of proinflammatory cytokines by flow cytometry and we found the AuNP interaction triggered transient secretion of tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma). In summary, we proved the biologically produced AuNPs entered all the tested cell types and triggered cell-specific responses. High AuNP uptake by tumor cells was related to decreased cell viability, while low nanoparticle uptake by fibroblasts triggered F-actin reorganization without remarkable toxicity. Thus, the biologically produced AuNPs hold promising potential as cancer drug carriers and likely require proper surface functionalization to shield phagocytizing cells. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2575
Počet záznamů: 1