Počet záznamů: 1
Physiological and transcriptome profiling of Chlorella sorokiniana: A study on azo dye wastewater decolorization
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SYSNO ASEP 0579195 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Physiological and transcriptome profiling of Chlorella sorokiniana: A study on azo dye wastewater decolorization Tvůrce(i) Tarbajová, V. (CZ)
Koláčková, M. (CZ)
Chaloupsky, P. (CZ)
Dobešová, M. (CZ)
Cápal, Petr (UEB-Q) RID, ORCID
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Švec, P. (CZ)
Skopalová Štěrbová, D. (CZ)
Vaculovičová, M. (CZ)
Richtera, L. (CZ)
Pérez-de-Mora, A. (DE)
Adam, V. (CZ)
Húska, D. (CZ)Celkový počet autorů 15 Číslo článku 132450 Zdroj.dok. Journal of Hazardous Materials. - : Elsevier - ISSN 0304-3894
Roč. 460, 15 October (2023)Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova pigment ; Microalgae ; bioremediation ; RNA-seq ; Raman micro-spectroscopy ; Fourier transform infrared spectroscopy Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora UPT-D - RVO:68081731 ; UEB-Q - RVO:61389030 UT WOS 001163085400001 EID SCOPUS 85170692338 DOI 10.1016/j.jhazmat.2023.132450 Anotace Over decades, synthetic dyes have become increasingly dominated by azo dyes posing a significant environmental risk due to their toxicity. Microalgae-based systems may offer an alternative for treatment of azo dye effluents to conventional physical-chemical methods. Here, microalgae were tested to decolorize industrial azo dye wastewater (ADW). Chlorella sorokiniana showed the highest decolorization efficiency in a preliminary screening test. Subsequently, the optimization of the experimental design resulted in 70% decolorization in a photobioreactor. Tolerance of this strain was evidenced using multiple approaches (growth and chlorophyll content assays, scanning electron microscopy (SEM), and antioxidant level measurements). Raman microspectroscopy was employed for the quantification of ADW-specific compounds accumulated by the microalgal biomass. Finally, RNA-seq revealed the transcriptome profile of C. sorokiniana exposed to ADW for 72 h. Activated DNA repair and primary metabolism provided sufficient energy for microalgal growth to overcome the adverse toxic conditions. Furthermore, several transporter genes, oxidoreductases-, and glycosyltransferases-encoding genes were upregulated to effectively sequestrate and detoxify the ADW. This work demonstrates the potential utilization of C. sorokiniana as a tolerant strain for industrial wastewater treatment, emphasizing the regulation of its molecular mechanisms to cope with unfavorable growth conditions. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0304389423017338
Počet záznamů: 1