Počet záznamů: 1
Solid phase extraction methods for purification of positively charged oligosaccharides and glycans
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SYSNO ASEP 0577618 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Solid phase extraction methods for purification of positively charged oligosaccharides and glycans Tvůrce(i) Smolková, Denisa (UIACH-O)
Moravcová, Dana (UIACH-O) RID, ORCID
Lavická, Jana (UIACH-O) ORCID, RIDCelkový počet autorů 3 Zdroj.dok. CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. - Brno : Ústav analytické chemie AV ČR, v. v. i., 2023 - ISBN 978-80-908154-0-7
S. 32-32Poč.str. 1 s. Forma vydání Online - E Akce CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./ Datum konání 23.10.2023 - 25.10.2023 Místo konání Brno Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova solid phase extraction ; oligosaccharides ; glycans Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA22-00236S GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 Anotace The crucial step for the analysis of oligosaccharides and glycans by capillary electrophoresis (CE) and/or mass spectrometry (CE-MS) is their derivatization by label. The signal of the unreacted label is routinely significantly higher, indicating the need to deplete the unreacted label prior to analysis by CE. The suitability of five different solid-phase extraction sorbents (Spe-ed Amide-2, Chromabond HILIC, HyperSep Diol, Bond Elut PBA, and HyperSep Hypercarb) was tested for the clean-up and concentration of positively labeled maltooligosaccharides from the reaction mixture. Maltooligosaccharides containing four to seven glucose units were labeled by cationic tags (2-aminoethyl)trimethylammonium chloride and (carboxymethyl)trimethylammonium chloride hydrazide (Girard’s reagent T). Purified samples were analyzed by CE with conductivity detection to compare the purification selectivity and efficiency using the tested SPE materials. The amide-based sorbent gave the best results and was used to process the N-linked glycans enzymatically released from the ribonuclease B sample. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2024 Elektronická adresa https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf
Počet záznamů: 1