Počet záznamů: 1
Non-specific effects of the CINNAMATE-4-HYDROXYLASE inhibitor piperonylic acid
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SYSNO ASEP 0575855 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Non-specific effects of the CINNAMATE-4-HYDROXYLASE inhibitor piperonylic acid Tvůrce(i) El Houari, I. (BE)
Klíma, Petr (UEB-Q) RID, ORCID
Baekelandt, A. (BE)
Staswick, P. (US)
Uzunova, V. (GB)
Del Genio, C. H. (GB)
Steenackers, W. (BE)
Dobrev, Petre (UEB-Q) RID, ORCID
Filepová, Roberta (UEB-Q) SAI
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Napier, R. (GB)
Petrášek, Jan (UEB-Q) RID, ORCID
Inzé, D. (BE)
Boerjan, W. (BE)
Vanholme, B. (BE)Celkový počet autorů 15 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 115, č. 2 (2023), s. 470-479Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova SALICYLIC-ACID ; ABSCISIC-ACID ; ARABIDOPSIS Obor OECD Biochemical research methods CEP EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000975691600001 EID SCOPUS 85156248679 DOI 10.1111/tpj.16237 Anotace Chemical inhibitors are often implemented for the functional characterization of genes to overcome the limitations associated with genetic approaches. Although it is well established that the specificity of the compound is key to success of a pharmacological approach, off-target effects are often overlooked or simply neglected in a complex biological setting. Here we illustrate the cause and implications of such secondary effects by focusing on piperonylic acid (PA), an inhibitor of CINNAMATE-4-HYDROXYLASE (C4H) that is frequently used to investigate the involvement of lignin during plant growth and development. When supplied to plants, we found that PA is recognized as a substrate by GRETCHEN HAGEN 3.6 (GH3.6), an amido synthetase involved in the formation of the indole-3-acetic acid (IAA) conjugate IAA-Asp. By competing for the same enzyme, PA interferes with IAA conjugation, resulting in an increase in IAA concentrations in the plant. In line with the broad substrate specificity of the GH3 family of enzymes, treatment with PA increased not only IAA levels but also those of other GH3-conjugated phytohormones, namely jasmonic acid and salicylic acid. Finally, we found that interference with the endogenous function of GH3s potentially contributes to phenotypes previously observed upon PA treatment. We conclude that deregulation of phytohormone homeostasis by surrogate occupation of the conjugation machinery in the plant is likely a general phenomenon when using chemical inhibitors. Our results hereby provide a novel and important basis for future reference in studies using chemical inhibitors. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2024 Elektronická adresa https://doi.org/10.1111/tpj.16237
Počet záznamů: 1