Počet záznamů: 1
Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field
- 1.
SYSNO ASEP 0574988 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field Tvůrce(i) Vahalová, Petra (URE-Y)
Havelka, Daniel (URE-Y) RID
Vaněčková, Eva (UFCH-W) SAI, ORCID
Zakar, Tomáš (URE-Y)
Kolivoška, Viliam (UFCH-W) RID, ORCID
Cifra, Michal (URE-Y) RID, ORCID, SAICelkový počet autorů 6 Číslo článku 133676 Zdroj.dok. Sensors and Actuators B - Chemical. - : Elsevier
Roč. 385, JUN 15 (2023)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Chemiluminescence ; Pulsed electric field ; Proteins ; Albumin ; Sensing ; Monitoring Vědní obor RIV BH - Optika, masery a lasery Obor OECD Optics (including laser optics and quantum optics) Vědní obor RIV – spolupráce Ústav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie CEP GX20-06873X GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora URE-Y - RVO:67985882 ; UFCH-W - RVO:61388955 UT WOS 000982222600001 EID SCOPUS 85150920169 DOI 10.1016/j.snb.2023.133676 Anotace Pulsed electric field (PEF) technology is an important emerging modality for both biomedicine and the food industry, yet the mechanisms of action of a PEF on a biomolecular level remain unclear. In order to gain a better understanding of this, a sensing platform was developed to enable sensitive detection of oxidative processes induced by the PEF. It was found that the application of PEF in phosphate buffer leads to the electrogeneration of reactive oxygen species (ROS) which in turn triggers intrinsic chemiluminescence. Furthermore, the presence of the bovine serum albumin (BSA) was found to increase the luminescence signal, which was attributed to biochemiluminescence due to the reactions of BSA with the electrogenerated ROS. A carbonyl determination assay was then used to confirm that the BSA molecules had indeed been oxidatively modified. We proposed a scheme describing the reactions leading from charge transfer at the anode/electrolyte interface to the actual photon emission, helping to elucidate the mechanisms of action of PEF on proteins. The results may to open up new avenues for the application of PEF technology. Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2024 Elektronická adresa DOI10.1016/j.snb.2023.133676
Počet záznamů: 1