Počet záznamů: 1
Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates
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SYSNO ASEP 0574261 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates Tvůrce(i) Ortiz, M. (ES)
Jauset-Rubio, M. (ES)
Trummer, O. (AT)
Foessl, I. (AT)
Kodr, David (UOCHB-X) ORCID
Acero, J. L. (ES)
Botero, M. L. (ES)
Biggs, P. (GB)
Lenartowicz, D. (GB)
Trajanoska, K. (NL)
Rivadeneira, F. (NL)
Hocek, Michal (UOCHB-X) RID, ORCID
Obermayer-Pietsch, B. (AT)
O'Sullivan, C. K. (ES)Zdroj.dok. ACS Central Science. - : American Chemical Society - ISSN 2374-7943
Roč. 9, č. 8 (2023), s. 1591-1602Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova bone mineral density ; chain reaction ; DNA Obor OECD Organic chemistry CEP GX20-00885X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 001032190400001 EID SCOPUS 85166762969 DOI 10.1021/acscentsci.3c00243 Anotace Osteoporosis is a multifactorial disease influenced by genetic and environmental factors, which contributes to an increased risk of bone fracture, but early diagnosis of this disease cannot be achieved using current techniques. We describe a generic platform for the targeted electrochemical genotyping of SNPs identified by genome-wide association studies to be associated with a genetic predisposition to osteoporosis. The platform exploits isothermal solid-phase primer elongation with ferrocene-labeled nucleoside triphosphates. Thiolated reverse primers designed for each SNP were immobilized on individual gold electrodes of an array. These primers are designed to hybridize to the SNP site at their 3′OH terminal, and primer elongation occurs only where there is 100% complementarity, facilitating the identification and heterozygosity of each SNP under interrogation. The platform was applied to real blood samples, which were thermally lysed and directly used without the need for DNA extraction or purification. The results were validated using Taqman SNP genotyping assays and Sanger sequencing. The assay is complete in just 15 min with a total cost of 0.3€ per electrode. The platform is completely generic and has immense potential for deployment at the point of need in an automated device for targeted SNP genotyping with the only required end-user intervention being sample addition. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2024 Elektronická adresa https://doi.org/10.1021/acscentsci.3c00243
Počet záznamů: 1