Počet záznamů: 1
Chemical Biopsy for GNMT as Noninvasive and Tumorigenesis-Relevant Diagnosis of Liver Cancer
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SYSNO ASEP 0568951 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chemical Biopsy for GNMT as Noninvasive and Tumorigenesis-Relevant Diagnosis of Liver Cancer Tvůrce(i) Oh, S. (KR)
Jo, S. (KR)
Kim, H. S. (KR)
Mai, V. (KR)
Endaya, B. (AU)
Neužil, Jiří (BTO-N) RID
Jung, K. H. (KR)
Hong, S. (KR)
Kim, J. (KR)
Park, S. (KR)Celkový počet autorů 10 Zdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 95, č. 2 (2023), s. 1184-1192Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova GLYCINE N-METHYLTRANSFERASE ; INFILTRATIVE HEPATOCELLULAR-CARCINOMA ; FOLATE-BINDING-PROTEIN Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora BTO-N - RVO:86652036 UT WOS 000923938900001 EID SCOPUS 85145985336 DOI 10.1021/acs.analchem.2c03944 Anotace Early diagnosis of hepatocellular carcinoma (HCC) is difficult, the patients burdened by life-long periodical examinations. Here, a new chemical biopsy approach was developed for noninvasive diagnosis of HCC using urine samples. Bioinformatic screening for tumor suppressors yielded glycine Nmethyltransferase (GNMT) as a biomarker with clinical relevance to HCC tumorigenesis. A liquid chromatography-mass spectrometry (LC-MS)-based chemical biopsy detecting nonradioactive 13C-sarcosine from 13C-glycine was designed to noninvasively assess liver GNMT activity extrahepatically. 13CSarcosine showed a strong correlation with GNMT in normal and cancerous liver cells. In an autochthonous animal model developing visible cancer nodules at 17 weeks, the urinary 13C-sarcosine chemical biopsy exhibited notable changes as early as 8 weeks, showing significant correlations with liver GNMT and molecular pathological changes. Our chemical biopsy approach should facilitate early and noninvasive diagnosis of HCC, with direct relevance to tumorigenesis, which can be straightforwardly applied to other diseases. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2024 Elektronická adresa https://pubs.acs.org/doi/full/10.1021/acs.analchem.2c03944
Počet záznamů: 1