Počet záznamů: 1

PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration

SYSNO ASEP	0568881
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration
Tvůrce(i)	Táborská, Johanka (UMCH-V) Blanquer, Andreu (FGU-C)_{RID, ORCID} Brynda, Eduard (UMCH-V)_RID Filová, Elena (FGU-C)_{RID, ORCID} Stiborová, Lenka (UMCH-V) Jenčová, V. (CZ) Havlíčková, K. (CZ) Riedelová, Zuzana (UMCH-V)_ORCID Riedel, Tomáš (UMCH-V)_{RID, ORCID}
Zdroj.dok.	International Journal of Nanomedicine. - : Dove Medical Press Roč. 18, 3 February (2023), s. 595-610
Poč.str.	16 s.
Jazyk dok.	eng - angličtina
Země vyd.	NZ - Nový Zéland
Klíč. slova	human platelet lysate ; diabetic ulcer ; fibrin coating
Vědní obor RIV	FB - Endokrinologie, diabetologie, metabolizmus, výživa
Obor OECD	Endocrinology and metabolism (including diabetes, hormones)
CEP	NV18-01-00332 GA MZd - Ministerstvo zdravotnictví
	LX22NPO5104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823
UT WOS	000928064400001
EID SCOPUS	85147762343
DOI	10.2147/IJN.S393890
Anotace	The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ϵ-caprolactone) and poly(ϵ-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2024
Elektronická adresa	https://www.dovepress.com/plclpcl-dressings-with-platelet-lysate-and-growth-factors-embedded-in--peer-reviewed-fulltext-article-IJN

Počet záznamů: 1