Počet záznamů: 1
Multiple roles of Pax6 in postnatal cornea development
- 1.
SYSNO ASEP 0568318 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Multiple roles of Pax6 in postnatal cornea development Tvůrce(i) Sunny, Sweetu Susan (UMG-J) ORCID
Láchová, Jitka (UMG-J)
Dupačová, Naoko (UMG-J)
Kozmik, Zbyněk (UMG-J) RIDZdroj.dok. Developmental Biology. - : Elsevier - ISSN 0012-1606
Roč. 491, Nov (2022), s. 1-12Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova eye ; development ; cornea ; pax6 ; conditional knockout ; E-cadherin ; Keratins Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP LM2018126 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0395 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA21-27364S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UMG-J - RVO:68378050 UT WOS 000911781200001 EID SCOPUS 85137301689 DOI 10.1016/j.ydbio.2022.08.006 Anotace Mammalian corneal development is a multistep process, including formation of the corneal epithelium (CE), endothelium and stroma during embryogenesis, followed by postnatal stratification of the epithelial layers and continuous renewal of the epithelium to replace the outermost corneal cells. Here, we employed the Cre-loxP system to conditionally deplete Pax6 proteins in two domains of ocular cells, i.e., the ocular surface epithelium (cornea, limbus and conjunctiva) (OSE) or postnatal CE via K14-cre or Aldh3-cre, respectively. Earlier and broader inactivation of Pax6 in the OSE resulted in thickened OSE with CE and limbal cells adopting the conjunctival keratin expression pattern. More restricted depletion of Pax6 in postnatal CE resulted in an abnormal cornea marked by reduced epithelial thickness despite increased epithelial cell proliferation. Immunofluorescence studies revealed loss of intermediate filament Cytokeratin 12 and diffused expression of adherens junction components, together with reduced tight junction protein, Zonula occludens-1. Furthermore, the expression of Cytokeratin 14, a basal cell marker in apical layers, indicates impaired differentiation of CE cells. Collectively, our data demonstrate that Pax6 is essential for maintaining proper differentiation and strong intercellular adhesion in postnatal CE cells, whereas limbal Pax6 is required to prevent the outgrowth of conjunctival cells to the cornea. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S0012160622001580?via%3Dihub
Počet záznamů: 1