Počet záznamů: 1
RNAseq analysis of brain aging in wild specimens of short-lived turquoise killifish: commonalities and differences with aging under laboratory conditions
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SYSNO ASEP 0564275 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název RNAseq analysis of brain aging in wild specimens of short-lived turquoise killifish: commonalities and differences with aging under laboratory conditions Tvůrce(i) Mazzetto, M. (DE)
Caterino, C. (DE)
Groth, M. (DE)
Ferrari, E. (IT)
Reichard, Martin (UBO-W) RID, ORCID, SAI, SAI
Baumgart, M. (DE)
Cellerino, A. (DE)Celkový počet autorů 7 Číslo článku msac219 Zdroj.dok. Molecular Biology and Evolution. - : Oxford University Press - ISSN 0737-4038
Roč. 39, č. 11 (2022)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova killifish ; Nothobranchius furzeri ; brain aging ; RNAseq ; gene expression Vědní obor RIV EG - Zoologie Obor OECD Zoology CEP GA19-01781S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UBO-W - RVO:68081766 UT WOS 000879850000007 EID SCOPUS 85141892367 DOI 10.1093/molbev/msac219 Anotace A vast body of studies is available that describe age-dependent gene expression in relation to aging in a number of different model species. These data were obtained from animals kept in conditions with reduced environmental challenges, abundant food, and deprivation of natural sensory stimulation. Here, we compared wild- and captive aging in the short-lived turquoise killifish (Nothobranchius furzeri). These fish inhabit temporary ponds in the African savannah. When the ponds are flooded, eggs hatch synchronously, enabling a precise timing of their individual and population age. We collected the brains of wild fish of different ages and quantified the global age-dependent regulation of transcripts using RNAseq. A major difference between captive and wild populations is that wild populations had unlimited access to food and hence grew to larger sizes and reached asymptotic size more rapidly, enabling the analysis of age-dependent gene expression without the confounding effect of adult brain growth. We found that the majority of differentially expressed genes show the same direction of regulation in wild and captive populations. However, a number of genes were regulated in opposite direction. Genes downregulated in the wild and upregulated in captivity were enriched for terms related to neuronal communication. Genes upregulated in the wild and downregulated in captive conditions were enriched in terms related to DNA replication. Finally, the rate of age-dependent gene regulation was higher in wild animals, suggesting a phenomenon of accelerated aging. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2023 Elektronická adresa https://academic.oup.com/mbe/article-pdf/39/11/msac219/46875255/msac219.pdf
Počet záznamů: 1