Počet záznamů: 1
Advantages of label free method in comparison with 2DE proteomic analysis of Butyrivibrio fibrisolvens 3071 grown on different carbon sources
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SYSNO ASEP 0563989 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Advantages of label free method in comparison with 2DE proteomic analysis of Butyrivibrio fibrisolvens 3071 grown on different carbon sources Tvůrce(i) Sechovcová, Hana (UZFG-Y) ORCID
Rudl Kulhavá, Lucie (FGU-C) ORCID, RID
Fliegerová, Kateřina (UZFG-Y) RID, ORCID
Killer, Jiří (UZFG-Y) RID, ORCID
Kopečný, Jan (UZFG-Y) RID, ORCIDZdroj.dok. Italian Journal of Animal Science - ISSN 1594-4077
Roč. 21, č. 1 (2022), s. 1508-1519Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. IT - Itálie Klíč. slova butyrivibrio fibrisolvens ; label-free ; 2DE ; proteomics ; rumen Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 ; FGU-C - RVO:67985823 UT WOS 000868161000001 EID SCOPUS 85148463551 DOI 10.1080/1828051X.2022.2129477 Anotace The aim of this study was the comparison of label-free method with 2DE to other analytical method of bacterium Butyrivibrio fibrisolvens extracellular protein samples. Label-free quantification (LF) method performed in this work was compared with previously obtained results of proteomic analysis using two-dimensional electrophoresis (2DE) and nano-liquid chromatography coupled with mass spectroscopy (nLC/MS). B. fibrisolvens as an important plant fibre degrader was cultivated on four different carbon sources (including xylan, xylose, glucose, and a mixture of xylan with glucose). The impact of growth substrate on protein profile was assessed by six pair-wise comparisons evaluating the significantly differently abundant extracellular proteins. Gel-free and gel-based methods resulted in substantially dissimilar results. The LC-MS/MS approach detected substrate-dependent differences in transport and binding membrane proteins (TBP) and nucleotidase, while the 2DE approach detected substrate effect on proteins included in protein synthesis and butyrate synthesis. On the other hand, both methods observed differentially regulated proteins involved in the glycolytic pathway, however, the only shared enzyme (protein detected both by 2DE and LC-MS/MS approach) was fructose-bisphosphate aldolase. The LC-MS/MS approach detected a high abundance of separated peptides. However, it cannot be easily considered as supreme to 2DE analysis. Both methods differ in sample preparation, their advantages and limitations. The study findings indicate these methods can complement each other and together they elucidate better the metabolic functions of B. fibrisolvens. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2023 Elektronická adresa https://www.tandfonline.com/doi/full/10.1080/1828051X.2022.2129477
Počet záznamů: 1