Počet záznamů: 1

Quaternized chitosan/heparin polyelectrolyte multilayer films for protein delivery

SYSNO ASEP	0563944
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Quaternized chitosan/heparin polyelectrolyte multilayer films for protein delivery
Tvůrce(i)	Urbaniak, Tomasz (UMCH-V) García-Briones, Gabriela S. (UMCH-V) Zhigunov, Alexander (UMCH-V)_{RID, ORCID} Hladysh, Sviatoslav (UMCH-V)_{RID, ORCID} Adrian, Edyta (UMCH-V)_{ORCID, RID} Lobaz, Volodymyr (UMCH-V)_{RID, ORCID} Krunclová, Tereza (UMCH-V)_ORCID Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID} Pop-Georgievski, Ognen (UMCH-V)_{RID, ORCID} Kubies, Dana (UMCH-V)_{RID, ORCID}
Zdroj.dok.	Biomacromolecules. - : American Chemical Society - ISSN 1525-7797 Roč. 23, č. 11 (2022), s. 4734-4748
Poč.str.	15 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	quaternized chitosan ; layer-by-layer film ; protein release
Vědní obor RIV	CD - Makromolekulární chemie
Obor OECD	Polymer science
CEP	GA20-08679S GA ČR - Grantová agentura ČR
	LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	UMCH-V - RVO:61389013
UT WOS	000886824100001
EID SCOPUS	85140966930
DOI	10.1021/acs.biomac.2c00926
Anotace	Layer-by-layer (LbL) polyelectrolyte coatings are intensively studied as reservoirs of bioactive proteins for modulating interactions between biomaterial surfaces and cells. Mild conditions for the incorporation of growth factors into delivery systems are required to maintain protein bioactivity. Here, we present LbL films composed of water-soluble N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride (HTCC), heparin (Hep), and tannic acid (TA) fabricated under physiological conditions with the ability to release heparin-binding proteins. Surface plasmon resonance analysis showed that the films formed on an anchoring HTCC/TA bilayer, with TA serving as a physical crosslinker, were more stable during their assembly, leading to increased film thickness and increased protein release. X-ray reflectivity measurements confirmed intermixing of the deposited layers. Protein release also increased when the proteins were present as an integral part of the Hep layers rather than as individual protein layers. The 4-week release pattern depended on the protein type, VEGF, CXCL12, and TGF-β1 exhibited a typical high initial release, whereas FGF-2 was sustainably released over 4 weeks. Notably, the films were nontoxic, and the released proteins retained their bioactivity, as demonstrated by the intensive chemotaxis of T-lymphocytes in response to the released CXCL12. Therefore, the proposed LbL films are promising biomaterial coating candidates for stimulating cellular responses.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2023
Elektronická adresa	https://pubs.acs.org/doi/10.1021/acs.biomac.2c00926

Počet záznamů: 1