Počet záznamů: 1
MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis
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SYSNO ASEP 0563830 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis Tvůrce(i) de Luxan-Hernandez, C. (DE)
Lohmann, J. (DE)
Tranque, E. (ES)
Chumová, Jana (MBU-M)
Binarová, Pavla (MBU-M) RID, ORCID
Salinas, J. (ES)
Weingartner, M. (DE)Číslo článku e202201507 Zdroj.dok. Life Science Alliance. - : Life Science Alliance
Roč. 6, č. 1 (2022)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova dna-damage response ; sr-related proteins ; u4/u6.u5 tri-snrnp ; lsm proteinsfunctional-analysisrnaidentificationspliceosomeatrphosphoproteomics ; lsm proteins ; functional-analysis ; rna ; identificationspliceosomeatrphosphoproteomics ; identification ; spliceosome ; atr ; phosphoproteomics Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000876974400001 EID SCOPUS 85140283164 DOI 10.26508/lsa.202201507 Anotace The coordination of cell division with stress response is essential for maintaining genome stability in plant meristems. Proteins involved in pre-mRNA splicing are important for these processes in animal and human cells. Based on its homology to the splicing factor SART1, which is implicated in the control of cell division and genome stability in human cells, we analyzed if MDF has similar functions in plants. We found that MDF associates with U4/U6.U5 tri-snRNP proteins and is essential for correct splicing of 2,037 transcripts. Loss of MDF function leads to cell division defects and cell death in meristems and was associated with up-regulation of stress-induced genes and down-regulation of mitotic regulators. In addition, the mdf-1 mutant is hypersensitive to DNA damage treatment supporting its role in coordinating stress response with cell division. Our analysis of a dephosphomutant of MDF suggested how its protein activity might be controlled. Our work uncovers the conserved function of a plant splicing factor and provides novel insight into the interplay of pre-mRNA processing and genome stability in plants. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.life-science-alliance.org/content/6/1/e202201507
Počet záznamů: 1