Počet záznamů: 1
H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin
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SYSNO ASEP 0563716 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin Tvůrce(i) de la Iglesia, O. (ES)
Jauregi, P. (ES)
Jodar, M. (ES)
Barrachina, F. (ES)
Děd, Lukáš (BTO-N) RID
Mallofre, C. (ES)
Rodriguez-Carunchio, L. (ES)
Manuel Corral, J. (ES)
Lluis Ballesca, J. (ES)
Komrsková, Kateřina (BTO-N) ORCID
Castillo, J. (ES)
Oliva, R. (ES)Celkový počet autorů 12 Číslo článku 12398 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 20 (2022)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova butyrylation ; acetylation ; spermatogenesis ; sperm ; sperm chromatin ; epigenetic regulation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GJ20-17403Y GA ČR - Grantová agentura ČR GC20-20217J GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000875419200001 DOI 10.3390/ijms232012398 Anotace Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play a crucial role in epigenetic control of nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on the same residue (H4K5bu) participates in temporal regulation of NH-NP transition in mice, delaying the bromodomain testis specific protein (BRDT)-dependent nucleosome disassembly and potentially marking retained nucleosomes. However, no information was available so far on this modification in human sperm. Here, we report a dual behavior of H4K5bu and H4K5ac in human normal spermatogenesis, suggesting a specific role of H4K5bu during spermatid elongation, coexisting with H4K5ac although with different starting points. This pattern is stable under different testicular pathologies, suggesting a highly conserved function of these modifications. Despite a drastic decrease of both PTMs in condensed spermatids, they are retained in ejaculated sperm, with 30% of non-colocalizing nucleosome clusters, which could reflect differential paternal genome retention. Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1422-0067/23/20/12398
Počet záznamů: 1