Počet záznamů: 1
Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit
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SYSNO ASEP 0562422 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit Tvůrce(i) Casanova-Sáez, R. (SE)
Mateo-Bonmatí, E. (SE)
Šimura, J. (CZ)
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Staswick, P. (US)
Ljung, K. (SE)Celkový počet autorů 7 Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 235, č. 1 (2022), s. 263-275Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis ; auxin ; drought ; gh3 ; salinity ; stress tolerance Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000782841700001 EID SCOPUS 85128219703 DOI 10.1111/nph.18114 Anotace Indole-3-acetic acid (IAA) controls a plethora of developmental processes. Thus, regulation of its concentration is of great relevance for plant performance. Cellular IAA concentration depends on its transport, biosynthesis and the various pathways for IAA inactivation, including oxidation and conjugation. Group II members of the GRETCHEN HAGEN 3 (GH3) gene family code for acyl acid amido synthetases catalysing the conjugation of IAA to amino acids. However, the high degree of functional redundancy among them has hampered thorough analysis of their roles in plant development. In this work, we generated an Arabidopsis gh3.1,2,3,4,5,6,9,17 (gh3oct) mutant to knock out the group II GH3 pathway. The gh3oct plants had an elaborated root architecture, showed an increased tolerance to different osmotic stresses, including an IAA-dependent tolerance to salinity, and were more tolerant to water deficit. Indole-3-acetic acid metabolite quantification in gh3oct plants suggested the existence of additional GH3-like enzymes in IAA metabolism. Moreover, our data suggested that 2-oxindole-3-acetic acid production depends, at least in part, on the GH3 pathway. Targeted stress-hormone analysis further suggested involvement of abscisic acid in the differential response to salinity of gh3oct plants. Taken together, our data provide new insights into the roles of group II GH3s in IAA metabolism and hormone-regulated plant development. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa https://doi.org/10.1111/nph.18114
Počet záznamů: 1