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A study of the interactions between human osteoblast-like cells and polymer composites with functionalized graphene derivatives using 2D correlation spectroscopy (2D-COS)
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SYSNO ASEP 0561736 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A study of the interactions between human osteoblast-like cells and polymer composites with functionalized graphene derivatives using 2D correlation spectroscopy (2D-COS) Tvůrce(i) Kolodziej, A. (PL)
Weselucha-Birczynska, A. (PL)
Dlugoń, E. (PL)
Swietek, Malgorzata Anna (UMCH-V) RID, ORCID
Gubernat, M. (PL)
Skalniak, L. (PL)
Blazewicz, M. (PL)Číslo článku 121862 Zdroj.dok. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. - : Elsevier - ISSN 1386-1425
Roč. 285, 15 January (2023)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova polymer nanocomposite ; poly(ε-caprolactone) (PCL) ; graphene oxide (GO) Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000860343500004 EID SCOPUS 85138073644 DOI 10.1016/j.saa.2022.121862 Anotace In response to the growing need for development of modern biomaterials for applications in regenerative medicine strategies, the research presented here investigated the biological potential of two types of polymer nanocomposites. Graphene oxide (GO) and partially reduced graphene oxide (rGO) were incorporated into a poly(ε-caprolactone) (PCL) matrix, creating PCL/GO and PCL/rGO nanocomposites in the form of membranes. Proliferation of osteoblast-like cells (human U-2 OS cell line) on the surface of the studied materials confirmed their biological activity. Fluorescence microscopy was able to distinguish the different patterns of interaction between cells (depending on the type of material) after 15 days of the test run. Raman micro-spectroscopy and two-dimensional correlation spectroscopy (2D-COS) applied to Raman spectra distinguished the nature of cell-material interactions after only 8 days. Combination of these two techniques (Raman micro-spectroscopy and 2D-COS analysis) facilitated identification of a much more complex cellular response (especially from proteins) on the surface of PCL/GO. The presented approach can be regarded as a method for early study of the bioactivity of membrane materials. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1386142522010101?via%3Dihub
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