Počet záznamů: 1
Age-related loss of axonal regeneration is reflected by the level of local translation
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SYSNO ASEP 0560416 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Age-related loss of axonal regeneration is reflected by the level of local translation Tvůrce(i) van Erp, S. (GB)
van Berkel, M. (NL)
Feenstra, E.M. (GB)
Sahoo, P. K. (US)
Wagstaff, L.J. (GB)
Twiss, J. L. (US)
Fawcett, James (UEM-P) ORCID
Eva, R. (GB)
Ffrench-Constant, Ch. (GB)Číslo článku 113594 Zdroj.dok. Experimental Neurology. - : Elsevier - ISSN 0014-4886
Roč. 339, may. (2021)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova axon regeneration ; human stem cells ; local translation ; proteomics ; in vitro live imaging ; axotomy Obor OECD Neurosciences (including psychophysiology Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000634819800002 EID SCOPUS 85100656511 DOI 10.1016/j.expneurol.2020.113594 Anotace Regeneration capacity is reduced as CNS axons mature. Using laser-mediated axotomy, proteomics and pummycin-based tagging of newly-synthesized proteins in a human embryonic stem cell-derived neuron culture system that allows isolation of axons from cell bodies, we show here that efficient regeneration in younger axons (d45 in culture) is associated with local axonal protein synthesis (local translation). Enhanced regeneration, promoted by co-culture with human glial precursor cells, is associated with increased axonal synthesis of proteins, including those constituting the translation machinery itself. Reduced regeneration, as occurs with the maturation of these axons by d65 in culture, correlates with reduced levels of axonal proteins involved in translation and an inability to respond by increased translation of regeneration promoting axonal mRNAs released from stress granules. Together, our results provide evidence that, as in development and in the PNS, local translation contributes to CNS axon regeneration. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0014488620304258?via%3Dihub
Počet záznamů: 1