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Protein coronas coating polymer-stabilized silver nanocolloids attenuate cytotoxicity with minor effects on antimicrobial performance
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SYSNO ASEP 0560415 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Protein coronas coating polymer-stabilized silver nanocolloids attenuate cytotoxicity with minor effects on antimicrobial performance Tvůrce(i) Batista, C. C. S. (BR)
Panico, K. (BR)
Trousil, Jiří (UMCH-V) RID, ORCID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
de Castro, C. E. (BR)
Štěpánek, Petr (UMCH-V) RID, ORCID
Giacomelli, F. C. (BR)Číslo článku 112778 Zdroj.dok. Colloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765
Roč. 218, October (2022)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova nano-bio interface ; silver colloids ; protein corona Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-materials (production and properties) Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000860770300006 EID SCOPUS 85136154636 DOI 10.1016/j.colsurfb.2022.112778 Anotace Silver nanoparticles are versatile platforms with a variety of applications in the biomedical field. In this framework, their presence in biological media inevitably leads to the interaction with proteins thus conducting to the formation of biomolecular coronas. This feature alters the identity of the nanomaterial and may affect many biological events. These considerations motivated the investigation of protein adsorption onto the surface of polymer-stabilized AgNPs. The metallic colloids were coated by polyethyleneimine (PEI), polyvinylpyrrolidone (PVP), and poly(2-vinyl pyridine)-b-poly(ethylene oxide) (PEO-b-P2VP), and nanoparticle-protein interaction was probed by using a library of analytical techniques. The experimental data revealed a higher extent of protein adsorption at the surface of AgNPs@PVP whereas PEO-b-P2VP coating conducted to the least amount. The main component of the protein coronas was evidenced to be bovine serum albumin (BSA), which is indeed the protein at the highest abundancy in the model biological media. We have further demonstrated reduced cytotoxicity of the silver colloids coated by biomolecular coronas as compared to the pristine counterparts. Nevertheless, the protein coatings did not notably reduce the antimicrobial performance of the polymer-stabilized AgNPs. Accordingly, although the protein-repelling property is frequently targeted towards longer in vivo circulation of nanoparticles, we herein underline that protein coatings, which are commonly treated as artifacts to be avoided, may indeed enhance the biological performance of nanomaterials. These findings are expected to be highly relevant in the design of polymer-stabilized metallic colloids intended to be used in healthcare. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0927776522004611?via%3Dihub
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