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Micro-electromembrane extraction through volatile free liquid membrane for the determination of β-lactam antibiotics in biological and environmental samples
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SYSNO ASEP 0560227 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Micro-electromembrane extraction through volatile free liquid membrane for the determination of β-lactam antibiotics in biological and environmental samples Tvůrce(i) Šlampová, Andrea (UIACH-O) RID
Kubáň, Pavel (UIACH-O) RID, ORCIDCelkový počet autorů 2 Číslo článku 123831 Zdroj.dok. Talanta. - : Elsevier - ISSN 0039-9140
Roč. 252, JAN (2023)Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova electromembrane extraction ; free liquid membrane ; volatile solvent Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000860344000007 EID SCOPUS 85136123626 DOI 10.1016/j.talanta.2022.123831 Anotace Micro-electromembrane extraction (μ-EME) was presented for the selective extraction of four main β-lactam antibiotics (penicillin, phenoxypenicillin, ampicillin, and amoxicillin) from complex samples. A volatile solvent (ethyl acetate or chloroform) was sandwiched between a plug of the complex sample and another plug of an aqueous acceptor solution in a transparent polymeric tube and formed the so-called free liquid membrane (FLM). The use of the FLM eliminated the evaporation of the solvent and enabled the μ-EME of the antibiotics, which was carried out by the application of DC voltage to the terminal aqueous solutions. The drugs in the complex sample were selectively transferred through the FLM to the acceptor solution, which was directly used for their determination by micellar electrokinetic chromatography with ultraviolet detection (MEKC-UV). The μ-EME was characterized by sub-μA electric currents, high elimination of matrix components, high stability of operational solutions, and suitability for extracting undiluted complex samples. The μ-EME/MEKC-UV method yielded good analytical repeatability (RSDs of peak areas ≤5%), extraction recoveries (40–84%), accuracy (92–105%) and linearity over one and a half order of magnitude (R2 ≥ 0.9998), and was applied to the determination of the four β-lactam antibiotics in human serum and waste water at clinically and environmentally relevant concentration levels. Further improvement in the method sensitivity was achieved by changing the μ-EME tube geometry
(conical shape) and increasing the complex sample volume (100 μL). The analytes were enriched by factors of 7.6–11.5, the limits of detection dropped down to less than 18 ng/mL, and the modified μ-EME/MEKC-UV method enabled the trace determination of β-lactam antibiotics in complex samples.Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2024 Elektronická adresa https://hdl.handle.net/11104/0333236
Počet záznamů: 1