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Chiral analysis of glycerol phosphates- can bacteria biosynthesize heterochiral phospholipid membranes?
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SYSNO ASEP 0559846 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chiral analysis of glycerol phosphates- can bacteria biosynthesize heterochiral phospholipid membranes? Tvůrce(i) Palyzová, Andrea (MBU-M) RID, ORCID
Guschina, Irina A. (GB)
Řezanka, Tomáš (MBU-M) ORCIDČíslo článku 463267 Zdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1676, AUG 2 2022 (2022)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Enantiomeric separation ; Glycerol phosphates ; rp-hplc ; MS-ESI plus ; Bacteria ; Heterochiral membranes Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000834927200005 EID SCOPUS 85132905685 DOI 10.1016/j.chroma.2022.463267 Anotace Phosphatidylglycerol (1,2-diacyl- sn-glycero-3-phospho-glycerol) (PG) is one of the most abundant lipids in bacteria. However, the chirality of the carbon atom on glycerol phosphate is different between the three kingdoms, Archaea, Bacteria, and Eukarya. Archaea membranes consist of phospholipids with glycerol1-phosphate (G1P) in the S configuration, whereas phospholipids of the other two kingdoms contain glycerol-3-phosphate (G3P) having R stereochemistry. In the present study, GC/MS and LC/MS methods sensitively detected G3P and G1P from four bacterial strains ( Bacillus amyloliquefaciens, B. subtilis, Clav-ibacter michiganensis, and Geobacillus stearothermophilus). Strain selection was carried out based on a GenBank search that revealed bacterial sequences associated with both enzymes involved in glycerolphosphate synthesis, i.e., glycerol-3-phosphate dehydrogenase and glycerol-1-phosphate dehydrogenase. The detection of G1P and G3P was made by comparing the retention times of synthetic standards with those of analyzed samples. The structures of both glycerol phosphates were confirmed by selected ion monitoring (SIM) at m/z 171.006. The total concentration of G3P and G1P was around 30 mu M, with a ratio of G3P to G1P of 4:1. We showed that PG was the most abundant phospholipid in all four bac-teria by using the following analytical techniques and chromatographic modes: hydrophilic interaction liquid chromatography (HILIC), reversed-phase high-performance liquid chromatography high-resolution electrospray ionization tandem mass spectrometry (RP-HPLC/HR-ESI tandem MS) in negative and positive ionization modes, and an enzymatic cleavage by phospholipase C. By using chiral chromatography, the presence of both enantiomers in the glycerol backbone of some molecular species of PG was revealed. These results allow us to conclude that the bacteria examined here produce both enantiomer glycerol phosphates.(c) 2022 Elsevier B.V. All rights reserved. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0021967322004605?via%3Dihub
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