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Nanodiamond-Quantum Sensors Reveal Temperature Variation Associated to Hippocampal Neurons Firing
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SYSNO ASEP 0559552 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nanodiamond-Quantum Sensors Reveal Temperature Variation Associated to Hippocampal Neurons Firing Tvůrce(i) Petrini, G. (IT)
Tomagra, G. (IT)
Bernardi, E. (IT)
Moreva, E. (IT)
Traina, P. (IT)
Marcantoni, A. (IT)
Picollo, F. (IT)
Kvaková, Klaudia (UOCHB-X) ORCID
Cígler, Petr (UOCHB-X) RID, ORCID
Degiovanni, I. P. (IT)
Carabelli, V. (IT)
Genovese, M. (IT)Číslo článku 2202014 Zdroj.dok. Advanced Science. - : Wiley
Roč. 9, č. 28 (2022)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova intracellular nanoscale sensing ; nanodiamonds ; nitrogen-vacancy (NV) centers ; ODMR Obor OECD Nano-materials (production and properties) CEP EF16_026/0008382 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000830459300001 EID SCOPUS 85134571799 DOI 10.1002/advs.202202014 Anotace Temperature is one of the most relevant parameters for the regulation of intracellular processes. Measuring localized subcellular temperature gradients is fundamental for a deeper understanding of cell function, such as the genesis of action potentials, and cell metabolism. Notwithstanding several proposed techniques, at the moment detection of temperature fluctuations at the subcellular level still represents an ongoing challenge. Here, for the first time, temperature variations (1 °C) associated with potentiation and inhibition of neuronal firing is detected, by exploiting a nanoscale thermometer based on optically detected magnetic resonance in nanodiamonds. The results demonstrate that nitrogen-vacancy centers in nanodiamonds provide a tool for assessing various levels of neuronal spiking activity, since they are suitable for monitoring different temperature variations, respectively, associated with the spontaneous firing of hippocampal neurons, the disinhibition of GABAergic transmission and the silencing of the network. Conjugated with the high sensitivity of this technique (in perspective sensitive to < 0.1 °C variations), nanodiamonds pave the way to a systematic study of the generation of localized temperature gradients under physiological and pathological conditions. Furthermore, they prompt further studies explaining in detail the physiological mechanism originating this effect. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2023 Elektronická adresa https://doi.org/10.1002/advs.202202014
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