Počet záznamů: 1
Photochemical synthesis of pink silver and its use for monitoring peptide nitration via surface enhanced Raman spectroscopy (SERS)
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SYSNO ASEP 0558956 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Photochemical synthesis of pink silver and its use for monitoring peptide nitration via surface enhanced Raman spectroscopy (SERS) Tvůrce(i) Sokolová, M. (CZ)
Šestáková, Hana (UOCHB-X)
Truksa, M. (CZ)
Šafařík, Martin (UOCHB-X) ORCID
Hadravová, Romana (UOCHB-X) RID, ORCID
Bouř, Petr (UOCHB-X) RID, ORCID
Šebestík, Jaroslav (UOCHB-X) RID, ORCIDZdroj.dok. Amino Acids. - : Springer - ISSN 0939-4451
Roč. 54, č. 9 (2022), s. 1261-1274Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. AT - Rakousko Klíč. slova nitration ; oxidative stress ; surface enhanced Raman spectroscopy (SERS) ; photochemical synthesis ; silver colloids ; posttranslational protein modification (PTM) Obor OECD Biophysics CEP EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA22-04669S GA ČR - Grantová agentura ČR Výzkumná infrastruktura e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000814499800002 EID SCOPUS 85132332193 DOI 10.1007/s00726-022-03178-w Anotace Oxidative stress may cause extended tyrosine posttranslational modifications of peptides and proteins. The 3-nitro-L-tyrosine (Nit), which is typically formed, affects protein behavior during neurodegenerative processes, such as Alzheimer’s and Parkinson’s diseases. Such metabolic products may be conveniently detected at very low concentrations by surface enhanced Raman spectroscopy (SERS). Previously, we have explored the SERS detection of the Nit NO2 bending vibrational bands in a presence of hydrogen chloride (Niederhafner et al., Amino Acids 53:517–532, 2021, ibid). In this article, we describe performance of a new SERS substrate, “pink silver”, synthesized photochemically. It provides SERS even without the HCl induction, and the acid further decreases the detection limit about 9 times. Strong SERS bands were observed in the asymmetric (1550–1475 cm−1) and symmetric (1360–1290 cm−1) NO stretching in the NO2 group. The bending vibration was relatively weak, but appeared stronger when HCl was added. The band assignments were supported by density functional theory modeling. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2023 Elektronická adresa https://doi.org/10.1007/s00726-022-03178-w
Počet záznamů: 1