Počet záznamů: 1
Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity
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SYSNO ASEP 0558860 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity Tvůrce(i) Obuca, Mina (UMG-J)
Cvačková, Zuzana (UMG-J) RID
Kubovčiak, Jan (UMG-J)
Kolář, Michal (UMG-J) RID, ORCID
Staněk, David (UMG-J) RIDCelkový počet autorů 5 Číslo článku e0265742 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 17, č. 4 (2022)Poč.str. 14 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova pre-messenger-rna ; atpase prp16 ; helix i ; spliceosome ; mechanism ; fidelity ; impairs ; gene Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP GC18-01911J GA ČR - Grantová agentura ČR EF16_019/0000785 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000804836900087 DOI 10.1371/journal.pone.0265742 Anotace Retinitis pigmentosa (RP) is a hereditary disease affecting tens of thousands of people world-wide. Here we analyzed the effect of an amino acid substitution in the RNA helicase DHX38 (Prp16) causing RP. DHX38 has been proposed as the helicase important for the 2(nd) step of splicing. We showed that DHX38 associates with key splicing factors involved in both splicing steps but did not find any evidence that the RP mutations changes DHX38 interaction profile with the spliceosome. We further downregulated DHX38 and monitored changes in splicing. We observed only minor perturbations of general splicing but detected modulation of ~70 alternative splicing events. Next, we probed DHX38 function in splicing of retina specific genes and found that FSCN2 splicing is dependent on DHX38. In addition, RHO splicing was inhibited specifically by expression of DHX38 RP variant. Finally, we showed that overexpression of DHX38 promotes usage of canonical as well as cryptic 5' splice sites in HBB splicing reporter. Together, our data show that DHX38 is a splicing factor that promotes splicing of cryptic splice sites and regulate alternative splicing. We further provide evidence that the RP-linked substitution G332D modulates DHX38 splicing activity. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265742
Počet záznamů: 1