Počet záznamů: 1
Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales
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SYSNO ASEP 0557609 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales Tvůrce(i) Tomasch, Jurgen (MBU-M) ORCID, RID
Ringel, V. (DE)
Wang, H. (DE)
Freese, H. (DE)
Bartling, P. (DE)
Brinkmann, H. (DE)
Vollmers, J. (DE)
Jarek, M. (DE)
Wagner-Doebler, I. (DE)
Petersen, J. (DE)Číslo článku 000787 Zdroj.dok. Microbial Genomics. - : Microbiology Society - ISSN 2057-5858
Roč. 8, č. 3 (2022)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova phaeobacter-gallaeciensis ; dinoroseobacter-shibae ; tropodithietic acid ; gen. nov. ; roseobacter ; genome ; phytoplankton ; adaptation ; bacteria ; agent ; bacteria-algae interaction ; conjugation ; horizontal gene transfer ; Roseobacter Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000788985200003 EID SCOPUS 85125871952 DOI 10.1099/mgen.0.000787 Anotace The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae. Here, we show that two large natural RepABCtype plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co- cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co- culture. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787
Počet záznamů: 1