Počet záznamů: 1

Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors

SYSNO ASEP	0557064
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors
Tvůrce(i)	Abbondanza, Alice (FGU-C)_ORCID Ribeiro Bas, Irina (FGU-C)_ORCID Modrák, Martin (MBU-M)_ORCID Čapek, Martin (FGU-C)_{RID, ORCID} Minich, Jessica (FGU-C) Tyshkevich, Alexandra (FGU-C) Naser, Shahed (FGU-C) Rangotis, Revan (FGU-C)_ORCID Houdek, Pavel (FGU-C)_ORCID Sumová, Alena (FGU-C)_{RID, ORCID} Dumas, S. (FR) Bernard, V. (FR) Janíčková, Helena (FGU-C)_{RID, ORCID, SAI}
Celkový počet autorů	13
Zdroj.dok.	Journal of Neuroscience. - : Society for Neuroscience - ISSN 0270-6474 Roč. 42, č. 13 (2022), s. 2786-2803
Poč.str.	18 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	acetylcholine ; behavior ; Fos ; interneurons ; nAChRs ; striatum
Obor OECD	Neurosciences (including psychophysiology
CEP	GJ19-07983Y GA ČR - Grantová agentura ČR
	LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	8J21FR022 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823 ; MBU-M - RVO:61388971
UT WOS	000779618200003
EID SCOPUS	85128160080
DOI	10.1523/JNEUROSCI.1627-21.2022
Anotace	Acetylcholine is an important modulator of striatal activity, and it is vital to controlling striatal-dependent behaviors, including motor and cognitive functions. Despite this significance, the mechanisms determining how acetylcholine impacts striatal signaling are still not fully understood. In particular, little is known about the role of nAChRs expressed by striatal interneurons. In the present study, we used FISH to determine which neuronal types express the most prevalent beta2 nicotinic subunit in the mouse striatum. Our data support a common view that nAChR expression is mostly restricted to striatal interneurons. Surprisingly though, cholinergic interneurons were identified as a population with the highest expression of beta2 nicotinic subunit. To investigate the functional significance of beta2containing nAChRs in striatal interneurons, we deleted them by injecting the AAV-Cre vector into the striatum of beta2-flox/flox male mice. The deletion led to alterations in several behavioral domains, namely, to an increased anxiety-like behavior, decrease in sociability ratio, deficit in discrimination learning, and increased amphetamine-induced hyperlocomotion and c-Fos expression in mice with beta2 deletion. Further colocalization analysis showed that the increased c-Fos expression was present in both medium spiny neurons and presumed striatal interneurons. The present study concludes that, despite being relatively rare, beta2-containing nAChRs are primarily expressed in striatal neurons by cholinergic interneurons and play a significant role in behavior.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2023
Elektronická adresa	https://doi.org/10.1523/JNEUROSCI.1627-21.2022

Počet záznamů: 1