Počet záznamů: 1

Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo

SYSNO ASEP	0556620
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo
Tvůrce(i)	Němčáková, Ivana (FGU-C)_{RID, ORCID} Litvinec, Andrej (FGU-C) Mandys, V. (CZ) Potocký, Štěpán (FZU-D)_{RID, ORCID} Plencner, Martin (FGU-C)_{RID, ORCID} Doubková, Martina (FGU-C)_{ORCID, RID} Naňka, O. (CZ) Olejníčková, Veronika (FGU-C)_{RID, ORCID, SAI} Šaňková, Barbora (FGU-C)_{RID, ORCID} Bartoš, M. (CZ) Ukraintsev, E. (CZ) Babčenko, O. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID} Kromka, Alexander (FZU-D)_{RID, ORCID, SAI} Rezek, B. (CZ) Sedmera, David (FGU-C)_{RID, ORCID, SAI}
Celkový počet autorů	16
Číslo článku	5264
Zdroj.dok.	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 12, č. 1 (2022)
Poč.str.	17 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	alkaline-phosphatase activity ; human osteogenic protein-1 ; mesenchymal stem-cells ; bone-formation ; surface functionalization ; antiadhesive properties ; metallic implants ; titanium implants ; dental implants
Obor OECD	Biomaterials (as related to medical implants, devices, sensors)
Vědní obor RIV – spolupráce	Fyzikální ústav - Chirurgie vč. transplantologie
CEP	GA21-06065S GA ČR - Grantová agentura ČR
	NV15-32497A GA MZd - Ministerstvo zdravotnictví
	LM2018110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF16_019/0000760 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Výzkumná infrastruktura	CzechNanoLab - 90110 - Vysoké učení technické v Brně
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823 ; FZU-D - RVO:68378271
UT WOS	000774204500044
EID SCOPUS	85127270573
DOI	10.1038/s41598-022-09183-z
Anotace	The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2023
Elektronická adresa	https://doi.org/10.1038/s41598-022-09183-z

Počet záznamů: 1